Recent advances in 3D cell culture and cellular differentiation, offer promising alternatives to study normal and pathological conditions in a human relevant context. 3D cell culture allows cells to grow and interact in a platform that simulates in vivo like conditions. Here, we describe the 3D self-assembly, and differentiation of human neural stem cells embedded in PASAP1 – a 100% animal-free synthetic matrix from Nanomed3D – grown on cellQART® Cell Culture Inserts made by SABEU.
Airway epithelial cells grown at the Air-liquid interface (ALI) are fundamental to test aerosolized drugs, measure the health effect of air pollutants and tobacco, model respiratory diseases and study infections like SARS-CoV-2, among others. Learn the basics of ALI cultures in this mini review!
TEER is a non-invasive technique to monitor the in vitro integrity of barrier tissue models grown on cell culture inserts. TEER assays help to elucidate mechanisms of barrier function, drug transport and to understand diseases where barrier tissues are compromised. Learn more about TEER here!
In this application report, InSCREENeX joins forces with cellQART® to develop a physiological and time-effective ALI model using InSCREENeX immortalised airway cells cultured on cellQART® Inserts. cellQART® Inserts allow optimal cultivation and better cell monitoring than the competitor.