Recent advances in 3D cell culture and cellular differentiation, offer promising alternatives to study normal and pathological conditions in a human relevant context. 3D cell culture allows cells to grow and interact in a platform that simulates in vivo like conditions. Here, we describe the 3D self-assembly, and differentiation of human neural stem cells embedded in PASAP1 – a 100% animal-free synthetic matrix from Nanomed3D – grown on cellQART® Cell Culture Inserts made by SABEU.
In this application report, InSCREENeX joins forces with cellQART® to develop a physiological and time-effective ALI model using InSCREENeX immortalised airway cells cultured on cellQART® Inserts. cellQART® Inserts allow optimal cultivation and better cell monitoring than the competitor.