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Assessment of hydrogel compatibility in cellQART® Inserts pre-loaded in Well Plates using casting or 3D printing approaches by Regenate 3D Bioprinter

Black Drop Biodrucker R&D team (thebioprinting.com), Karina Cuanalo-Contreras, PhD

The potential suitability of two different hydrogels (agarose and recombinant animal-free collagen) for tissue engineering applications was tested using cellQART® Cell Culture Inserts pre-loaded in 24 Well Plates. For that, hydrogels were directly casted or 3D printed (drop-on-demand technique) into the Cell Culture Insert Membranes to achieve a cylinder-like structure using the Regenate 3D Bioprinter from Black Drop Biodrucker. Drop deposition, hydrogel shape and preliminary cellular compatibility was assessed. Either agarose or recombinant collagen demonstrated promising performance, independently of the production method. Cell assays revealed high proliferation rates of PDLSCs on the Cell Culture Insert Membrane surface. Design features and membrane parameter consistency of cellQART® Inserts supported operational efficiency and experimental reproducibility. All hydrogels are currently undergoing further optimization for specific application needs. Due to confidential reasons, some data cannot be disclosed.


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Self-assembly of human neural 3D cell cultures
using Nanomed3D PASAP1 hydrogel and cellQART® Cell Culture Inserts

Nanomed3D R&D team (nanomed3d.com), Karina Cuanalo-Contreras, PhD

Recent advances in 3D cell culture and cellular differentiation, offer promising alternatives to study normal and pathological conditions in a human relevant context. 3D cell culture allows cells to grow and interact in a platform that simulates in vivo like conditions. Here, we describe the 3D self-assembly, and differentiation of human neural stem cells embedded in PASAP1 – a 100% animal-free synthetic matrix from Nanomed3D – grown on cellQART® Cell Culture Inserts made by SABEU.


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cellZscope® TEER systems are 100% compatible with cellQART® Inserts

nanoAnalytics GmbH (nanoanalytics.com)

cellZscope® is an innovative automated TEER measurement system that puts an end to labour and time consuming manual TEER methods. We are thrilled to announce that cellQART® Inserts are fully compatible with cellZscope® systems.


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Optimized air-liquid-interface respiratory tract model using InSCREENeX cells and cellQART® Inserts

Dr. Kristina Nehlsen and Dr. Tobias May | InSCREENeX GmbH (inscreenex.de)

In this application report, InSCREENeX joins forces with cellQART® to develop a physiological and time-effective ALI model using InSCREENeX immortalised airway cells cultured on cellQART® Inserts. cellQART® Inserts allow optimal cultivation and better cell monitoring than the competitor.


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