Optimized air-liquid-interface respiratory tract model using InSCREENeX cells and cellQART® Inserts
Prepared by: Dr. Kristina Nehlsen and Dr. Tobias May
InSCREENeX GmbH (inscreenex.de)
The development of efficient and physiological relevant airway tract models is a challenge in the field.
Here, InSCREENeX and cellQART® describe the generation of an optimized airway model in an air-liquid-interface culture. The model portrays tight junctions as well as cell polarisation. Superior TEER measurements confirm tight barrier formation. In addition, epithelial barrier establishment time is reduced by 2-fold, lowering maintenance costs without compromising integrity or function.
This innovative model was developed using InSCREENeX immortalised upper and lower airway epithelial cells cultured in FasTEER differentiation medium on cellQART® Cell Culture Inserts. The application report can be downloaded right here.